Comparative detection of Giardia lamblia by immunochromatographic assay from stool samples in Erbil Province
DOI:
https://doi.org/10.25212/lfu.qzj.9.4.43Keywords:
Giardia lamblia, Immunochromatographic assay, Protozoa, Parasite, Light microscope.Abstract
Background: A flagellated protozoan parasite Giardia lamblia is one of the most significant causes of parasitic gastrointestinal illnesses. The main method of transmission is through the fecal-oral route, and the life cycle is straightforward and direct, including the motile trophozoite and nonmotile cyst stages. The aim of the study was to compare microscopy and immunochromatographic tests for diagnosing Giardia lamblia. Methods: This cross-sectional study was done by selecting 100 microscopically negative samples from groups of people In Erbil city, which were collected and stored at -20 °C for further testing. The result of the study conducted showed that, from 100 frozen samples, 3% were positive by immunochromatographic rapid testing. Males were more likely to participate in this study because most of the participants were food handlers. There was no statistically significant association between the gastrointestinal symptoms of positive patients; 1 (2.8%) has symptoms, and 2 (3.1%) are asymptomatic. It is concluded that Immunochromatographic assays are easy to perform, rapid, sensitive, and specific. It showed that the test may be useful in the diagnosis of infection in large cases or in studying the epidemiology of Giardia lamblia
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Abbas, N. F., El-Shaikh, K. A., & Almohammady, M. (2011). Prevalence of Giardia lamblia in diarrheic children in Almadinah Almunawarh, KSA. 5, 25-30.
Al-Mekhlafi, H. (2017). Giardia duodenalis infection among rural communities in Yemen: A community-based assessment of the prevalence and associated risk factors. 10(10), 987-995.
Ayeh-Kumi, P., Quarcoo, S., Kwakye-Nuako, G., Kretchy, J., Osafo-Kantanka, A., Mortu, S.(2009). Prevalence of intestinal parasitic infections among food vendors in Accra, Ghana. 32(1), 1-8.
Barazesh, A., Majidi, J., Fallah, E., Jamali, R., Abdolalizade, J., & Gholikhani, R. (2010). Designing of enzyme linked immunosorbent assay (ELISA) kit for diagnosis copro-antigens of Giardia lamblia. 9(31), 5025-5027.
Belkessa, S., Ait-Salem, E., Laatamna, A., Houali, K., Sönksen, U. W., Hakem, A. (2021). Prevalence and clinical manifestations of Giardia intestinalis and other intestinal parasites in children and adults in Algeria. 104(3), 910.
Cama, V. A., & Mathison, B. A. J. C. i. L. M. (2015). Infections by intestinal coccidia and Giardia duodenalis. 35(2), 423-444.
Choy, S. H., Al-Mekhlafi, H. M., Mahdy, M. A., Nasr, N. N., Sulaiman, M., Lim, Y. A., & Surin, J. (2014). Prevalence and associated risk factors of Giardia infection among indigenous communities in rural Malaysia. 4(1), 6909.
Duque-Beltrán, S., Nicholls-Orejuela, R. S., Arévalo-Jamaica, A., Guerrero-Lozano, R., Montenegro, S., & James, M. (2002). Detection of Giardia duodenalis antigen in human fecal eluates by enzyme-linked immunosorbent assay using polyclonal antibodies. 97, 1165-1168.
Faubert, G. (2000). Immune response to Giardia duodenalis. 13(1), 35-54.
Feng, Y., & Xiao, L. (2011). Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. 24(1), 110-140.
Fink, M, Shapiro, D., & Singer, S. (2020). Giardia lamblia: Laboratory maintenance, lifecycle induction, and infection of murine models. 57(1), e102.
Garcia, L. S., Shimizu, R. Y., Novak, S., Carroll, M., & Chan, F. (2003). Commercial assay for detection of Giardia lamblia and Cryptosporidium parvum antigens in human fecal specimens by rapid solid-phase qualitative immunochromatography. 41(1), 209-212.
Guimarães, S., & Sogayar, M. (2002). Detection of anti-Giardia lamblia serum antibody among children of day care centers. 36, 63-68.
Hanson, K. L., & Cartwright, C.(2001). Use of an enzyme immunoassay does not eliminate the need to analyze multiple stool specimens for sensitive detection of Giardia lamblia. 39(2), 474-477.
Heyworth, M. (2014). Diagnostic testing for Giardia infections. 108(3), 123-125.
Jahan, N., Khatoon, R., Ahmad, S. (2014). A comparison of microscopy and enzyme linked immunosorbent assay for diagnosis of Giardia lamblia in human faecal specimens. 8(11), DC04.
Jelinek, T., & Neifer, S. (2013). Detection of Giardia lamblia stool samples: a comparison of two enzyme-linked immunosorbent assays. 2, 39.
khudair Hussein, T. (2010). Prevalence and related risk factors for Giardia lamblia infection among children with acute diarrhea in thi-qar, southern Iraq. 4(4), 68-74.
Kotloff, K. L., Nataro, J. P., Blackwelder, W. C., Nasrin, D., Farag, T. H., Panchalingam, S., . . . Breiman, R. (2013). Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. 382(9888), 209-222.
Muhsen, K., & Levine, M. (2012). A systematic review and meta-analysis of the association between Giardia lamblia and endemic pediatric diarrhea in developing countries. 55(suppl_4), S271-S293.
Pestechian, N., Rasekh, H., Rostami-Nejad, M., Yousofi, H. A., Hosseini-Safa, A. J. G., & bench, H. (2014). Molecular identification of Giardia lamblia; is there any correlation between diarrhea and genotyping in Iranian population? , 7(3), 168.
Platts-Mills, J. A., Operario, D. J., & Houpt, E.(2012). Molecular diagnosis of diarrhea: current status and future potential. 14, 41-46.
Ryan, U., & Cacciò, S. (2013). Zoonotic potential of Giardia. 43(12-13), 943-956.
Samie, A., Tanih, N. F., Seisa, I., Seheri, M., Mphahlele, J., ElBakri, A., . . . control. (2020). Prevalence and genetic characterization of Giardia lamblia in relation to diarrhea in Limpopo and Gauteng provinces, South Africa. 9, e00140.
Selim, M. A. E.-W., Taha, A. A. E.-R., Abd El-Aal, N. F., Farag, T. I., & Yousef, A. (2015). DETECTION OF GIARDIA INTESTINALIS COPROANTIGENS IN DIARRHEIC SAMPLES BY IMMUNOCHROMATOGRAPHIC AND ELISATECHNIQUES. 45(2), 273-283.
Solaymani-Mohammadi, S., & Singer, S. (2010). Giardia duodenalis: the double-edged sword of immune responses in giardiasis. 126(3), 292-297.
Sorell, L., Garrote, J. A., Galvan, J. A., Velazco, C., Edrosa, C. R., Arranz, E. (2004). Celiac disease diagnosis in patients with giardiasis: high value of antitransglutaminase antibodies. 99(7), 1330-1332.
VIESY, S., ABDI, J., REZAEI, Z., FEIZI, J. (2020). Evaluation of the Prevalence of Giardia Infection in People Referred to the Laboratories of Ilam City. 14(6).
Weitzel, T., Dittrich, S., Möhl, I., Adusu, E., Jelinek, T. (2006). Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samples.
(7), 656-659.
Alharbi, A., Toulah, F.., Wakid, M., Azhar, E., Farraj, S., & Mirza, A. (2020). Detection of Giardia lamblia by microscopic examination, rapid chromatographic immunoassay test, and molecular technique. 12 -(9)
-Ghieth, M. Kotb, M. Abu-Sarea, E, & El-Badry, A. (2016). Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals. 40, 1470-1474.
Hooshyar, H., Rostamkhani, P., Arbabi, M., Delavari, M., & Bench, H.(2019). Giardia lamblia infection: review of current diagnostic strategies.
Krumrie, S., Capewell, P., Smith-Palmer, A., Mellor, D., Weir, W., Alexander, C. (2022).
A scoping review of risk factors and transmission routes associated with human giardiasis outbreaks in high-income settings. 2, 100084.
Singer, S., Fink, M., & Angelova, V. (2019). Recent insights into innate and adaptive immune responses to Giardia. 106, 171-208.
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